Composite

Part:BBa_K2926285:Design

Designed by: Astrid T�bber   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-21)


Basic Insert to express Cas13a (Lsh) and a guideRNA array in S. cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5277
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5302
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1066
    Illegal BglII site found at 1612
    Illegal BglII site found at 1690
    Illegal BglII site found at 2377
    Illegal BglII site found at 3265
    Illegal BglII site found at 4255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5277
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5277
    Illegal AgeI site found at 105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 833
    Illegal SapI.rc site found at 1158
    Illegal SapI.rc site found at 2028
    Illegal SapI.rc site found at 2862


Design Notes

bpjvsym


Source

avdln ,

References